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KMID : 1123920080220040903
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Korean Journal of Oriental Physiology and Pathology
2008 Volume.22 No. 4 p.903 ~ p.906
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Radical Scavenging Activity of Gallic Acid from Woodfordia Fruticosa Flowers
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Bhatt Lok Ranjan
Yook Chan-Nam
Choi Hwa-Jung
Baek Seung-Hwa
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Abstract
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Woodfordia fruticosa Kurz (Lythraceae) is used in the treatment of various ailments in traditional medicines. DPPH activity guided fractionation and purification process was used to identify the free radical-scavenging components from the flowers of this plant. The methanolic extract of the plant was first fractionated into four extracts; namely, n-hexane, chloroform, ethyl acetate and water fractions. Among them, the ethyl acetate fraction was found to be the most effective and was further subjected to activity guided-fractionation and isolation procedures. After successive column chromatography on silica gel and Sephadex LH-20, gallic acid, which is responsible for the radical scavenging activity, was isolated and its structure was elucidated by spectral methods ( NMR, NMR) and by comparison with literature.
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KEYWORD
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Woodfordia fruticosa, antioxidants, gallic acid, DPPH, HPLC
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